Column:

HP-C18 , 5 µm, 120 Å, 4.6 ×150 mm

Mobile phase:

0.10 M Phosphate buffer, pH 3.1

Flow rate:

1.0 mL/min

Temperature:

23 ^oC

Detection:

UV 210 nm

Injection volume:

5 µL

Sample:

1. Formic Acid（蚁酸,10 mM）,
2. Malic Acid（苹果酸, 10 mM）,
3. Lactic Acid（乳酸,10 mM）,
4. Acetic Acid（乙酸, 10 mM）,
5. Citric Acid（柠檬酸, 10 mM）,
6. Succinic Acid（琥珀酸,10 mM）